Optimizing Fermentation Control for B.Licheniformis

Researcher: Ola Johnsson, Tore Hägglund (Area leader), and Charlotta Johnsson (vice area-leader)

Start-date: 2010-08

End-date: 2011-07

Results: Today, the project has financial support through the PIC-LU project (Process ndustrial Centre Lund University, supported by SSF, start date 2011-07), and is perfomed in close collaboration with Novozymes A/S.

Acknowledgement: Thanks to LCCC for providing means in the startup of the project, a total of 11 months. 




The project will focus on developing, improving and optimizing fermentation control strategies for the B. licheniformis fed batch processes. This process can produce vast amounts of protein but is sensitive to overdosing and process disturbances which lead to process variations and possibly crashed fermentations. There is thus a strong motivation for developing more robust control strategies for this process. The project aim is to develop a general method for finding optimal control strategies for various B. licheniformis production strains.

In addition to developing and evaluating bioprocess control strategies which can improve robustness and yield of specificB. licheniformis processes, it is also of interest that the developed methods are general enough to allow for implementation on various enzyme-producing processes utilising different B. licheniformis strains. This will require the identification of key physiological variables in the strains and an understanding of the interaction between these properties and the way the process is controlled.

This project is performed in collaboration with Novozymes AS.